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One of many examples is Matsunaga Sekigo’s (1592–1657) , 1640), which opens by stating, “Between heaven and earth, there are three major ways: the Confucian, which is the way of Confucius; the Buddhist, which is the way of Shakyamuni; and the Daoist, which is the way of Laozi.” The most commonly used references to Confucianism in Japanese history, traditional and modern, are the terms , literally referring to “weaklings.” The latter was a reference to scholars who tended to work with their minds rather than their bodies and were, as a result, perceived as being weaker. Acceptance of the term partly reflected Confucius’ distaste for coercive force as opposed to the softer power of ethical examples and the allegedly irresistible efficacy of moral suasion.The term was used by later scholars in explaining the teachings of Confucius (551–479 B. In the west, the term “Confucianism” first came into use following contacts between Jesuit missionaries and Chinese scholars.In this respect, Confucianism was the secular philosophy operative in the ordinary world of everyday existence, at one level or another, throughout Japanese history, well into modern times.As often as not, however, its teachings became so integrated into Japanese culture without appeal to their overall identity as “Confucian” teachings, leading many, over time, to assume naively that they were integral to the Japanese mind and its myriad expressions in history and culture.Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite.However, some cell lines become immortal through a process called transformation, which can occur spontaneously or can be chemically or virally induced.In this way, the foundations of Confucianism as intellectual discourse are hopefully laid bare. There was no single word in the traditional East Asian lexicon that corresponded exactly and consistently to “Confucianism,” yet by late-medieval and early-modern times, there was a fairly distinct understanding among Japanese intellectuals of an essential unity to the diverse Confucian teachings otherwise variously referred to.This is clear in references to the “three teachings”, Confucianism, Buddhism, and Daoism (and/or Shintō).

Minus the honorific , Confucius’ name was Kong Qiu.If a surplus of cells are available from subculturing, they should be treated with the appropriate protective agent (e.g., DMSO or glycerol) and stored at temperatures below –130°C (cryopreservation) until they are needed.For more information on subculturing and cryopreserving cells, refer to the Guidelines for Maintaining Cultured Cells.Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment.The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established.

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If a subpopulation of a cell line is positively selected from the culture by cloning or some other method, this cell line becomes a cell strain.

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